Comparative analysis of drought responsive transcriptome in Brassica napus genotypes with contrasting drought tolerance under different potassium levels

Drought is a major limiting factor of Brassica napus (rapeseed) and potassium (K) plays important roles in rapeseed drought tolerance. Previous studies have reported that cultivars characterized by different K status showed contrasting However, the molecular mechanism underlying tolerance remains unclear. In this study, comparative transcriptome analysis was conducted between drought-tolerant cultivar Youyan57 drought-sensitive Chuanyou36 exposed to PEG6000 simulated stress with two levels (1.0 0.01 mM K2SO4, referred NK LK, respectively). A total 1689 differentially expressed genes (DEGs) were identified at NK. DEGs involved photosynthesis, glutathione biosynthesis, IAA signal transduction up-regulated By contrast, down-regulated significantly enriched biosynthesis amino acids, cysteine methionine metabolism glucosinolate biosynthesis. Transcription profile affected seriously LK treatment since only 1050 identified. acids reduced largely. Furthermore, conspicuous up-regulation protein phosphatase 2 C could lead more severe which negatively participated abscisic acid (ABA) transduction. Taken together, set drought-regulated several pathways, provided information about mechanisms